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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has long been noticed in several tumors, which results in chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial part in marketing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are already described. Two more splice variants were reported but comprehensive nucleotide sequence facts isn't nevertheless offered.

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Blocking peptides are peptides that bind particularly into the concentrate on antibody and block antibody binding. These peptide ordinarily incorporates the epitope identified from the antibody.

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This guideline outlines further more probable optimization of mobile and immunoassay parameters to be sure the best possible results are...

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This guide outlines further more probable optimization of mobile and immunoassay parameters to make certain the very best final results are...

Antibodies certain to the blocking peptide now not bind towards the epitope around the goal protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is precise.

Focus on Details Cyclin E belongs towards the very conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation designs which add to your temporal coordination of every mitotic occasion. Cyclin E sorts a posh with and capabilities being a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates in the G1-S phase boundary and is also degraded as cells progress by means of S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...

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